Preconcentration of trace amounts of lorazepam in aqueous samples by functionalizing graphene oxide with 2-aminopyridine

Document Type : Original Article

Authors

• ali moghimi ¹
• Milad Abniki ²

¹ Department of Chemistry, Faculty o f pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

² PhD in Analytical Chemistry, Department of Resins and Additives, Institute for Color Science and Technology

Abstract

In this study, an approach was used to preconcentrate lorazepam in water samples. This study has tried to present a new method of solid phase diffusion extraction of small amounts of pharmaceutical effluents as an indicator of Lorazepam drug in aqueous samples by graphene oxide with 2-aminopyridine and its measurement by visible and ultraviolet spectroscopy in real samples. This method includes a two-phase system in which the donor phase of the aqueous sample contains lorazepam and the acceptor phase is functionalized graphene oxide. Experiments were performed in two stages of extracting water samples including lorazepam drug desorption using ammonia solvent and the desorbed samples were submitted to UV-Vis spectrophotometer for further analysis. This method is cheap, simple and fast, and it is also a sensitive and reliable method for extracting, preconcentrating and measuring small amounts of lorazepam. The effective parameters on drug extraction, which include: the effect of pH, the amount and type of detergent solvent, and the extraction time, have been investigated. The concentration factor is 19, the detection limit of the method is 1.1 µg/L, and the relative standard deviation is 2.17%.

Keywords

• Graphene oxide
• 2-aminopyridine
• UV-Vis spectrophotometery
• Lorazepam drug

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